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Page 1
High-speed fluorescence image-enabled cell sorting.
Schraivogel D, Kuhn TM, Rauscher B, Rodríguez-Martínez M, Paulsen M, Owsley K, Middlebrook A, Tischer C, Ramasz B, Ordoñez-Rueda D, Dees M, Cuylen-Haering S, Diebold E, Steinmetz LM. Schraivogel D, et al. Science. 2022 Jan 21;375(6578):315-320. doi: 10.1126/science.abj3013. Epub 2022 Jan 20. Science. 2022. PMID: 35050652 Free PMC article.
A global genetic interaction network maps a wiring diagram of cellular function.
Costanzo M, VanderSluis B, Koch EN, Baryshnikova A, Pons C, Tan G, Wang W, Usaj M, Hanchard J, Lee SD, Pelechano V, Styles EB, Billmann M, van Leeuwen J, van Dyk N, Lin ZY, Kuzmin E, Nelson J, Piotrowski JS, Srikumar T, Bahr S, Chen Y, Deshpande R, Kurat CF, Li SC, Li Z, Usaj MM, Okada H, Pascoe N, San Luis BJ, Sharifpoor S, Shuteriqi E, Simpkins SW, Snider J, Suresh HG, Tan Y, Zhu H, Malod-Dognin N, Janjic V, Przulj N, Troyanskaya OG, Stagljar I, Xia T, Ohya Y, Gingras AC, Raught B, Boutros M, Steinmetz LM, Moore CL, Rosebrock AP, Caudy AA, Myers CL, Andrews B, Boone C. Costanzo M, et al. Science. 2016 Sep 23;353(6306):aaf1420. doi: 10.1126/science.aaf1420. Science. 2016. PMID: 27708008 Free PMC article.
Targeted Perturb-seq enables genome-scale genetic screens in single cells.
Schraivogel D, Gschwind AR, Milbank JH, Leonce DR, Jakob P, Mathur L, Korbel JO, Merten CA, Velten L, Steinmetz LM. Schraivogel D, et al. Nat Methods. 2020 Jun;17(6):629-635. doi: 10.1038/s41592-020-0837-5. Epub 2020 Jun 1. Nat Methods. 2020. PMID: 32483332 Free PMC article.
Multiplexed precision genome editing with trackable genomic barcodes in yeast.
Roy KR, Smith JD, Vonesch SC, Lin G, Tu CS, Lederer AR, Chu A, Suresh S, Nguyen M, Horecka J, Tripathi A, Burnett WT, Morgan MA, Schulz J, Orsley KM, Wei W, Aiyar RS, Davis RW, Bankaitis VA, Haber JE, Salit ML, St Onge RP, Steinmetz LM. Roy KR, et al. Nat Biotechnol. 2018 Jul;36(6):512-520. doi: 10.1038/nbt.4137. Epub 2018 May 7. Nat Biotechnol. 2018. PMID: 29734294 Free PMC article.
The cellular growth rate controls overall mRNA turnover, and modulates either transcription or degradation rates of particular gene regulons.
García-Martínez J, Delgado-Ramos L, Ayala G, Pelechano V, Medina DA, Carrasco F, González R, Andrés-León E, Steinmetz L, Warringer J, Chávez S, Pérez-Ortín JE. García-Martínez J, et al. Nucleic Acids Res. 2016 May 5;44(8):3643-58. doi: 10.1093/nar/gkv1512. Epub 2015 Dec 29. Nucleic Acids Res. 2016. PMID: 26717982 Free PMC article.