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DNA interference states of the hypercompact CRISPR-CasΦ effector.
Pausch P, Soczek KM, Herbst DA, Tsuchida CA, Al-Shayeb B, Banfield JF, Nogales E, Doudna JA. Pausch P, et al. Nat Struct Mol Biol. 2021 Aug;28(8):652-661. doi: 10.1038/s41594-021-00632-3. Epub 2021 Aug 11. Nat Struct Mol Biol. 2021. PMID: 34381246 Free PMC article.
Targeted nonviral delivery of genome editors in vivo.
Tsuchida CA, Wasko KM, Hamilton JR, Doudna JA. Tsuchida CA, et al. Proc Natl Acad Sci U S A. 2024 Mar 12;121(11):e2307796121. doi: 10.1073/pnas.2307796121. Epub 2024 Mar 4. Proc Natl Acad Sci U S A. 2024. PMID: 38437567 Free PMC article. Review.
Chimeric CRISPR-CasX enzymes and guide RNAs for improved genome editing activity.
Tsuchida CA, Zhang S, Doost MS, Zhao Y, Wang J, O'Brien E, Fang H, Li CP, Li D, Hai ZY, Chuck J, Brötzmann J, Vartoumian A, Burstein D, Chen XW, Nogales E, Doudna JA, Liu JG. Tsuchida CA, et al. Mol Cell. 2022 Mar 17;82(6):1199-1209.e6. doi: 10.1016/j.molcel.2022.02.002. Epub 2022 Feb 25. Mol Cell. 2022. PMID: 35219382 Free PMC article.
Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells.
Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Hofman L, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, Doudna JA. Tsuchida CA, et al. Cell. 2023 Oct 12;186(21):4567-4582.e20. doi: 10.1016/j.cell.2023.08.041. Epub 2023 Oct 3. Cell. 2023. PMID: 37794590 Free PMC article.
CasPEDIA Database: a functional classification system for class 2 CRISPR-Cas enzymes.
Adler BA, Trinidad MI, Bellieny-Rabelo D, Zhang E, Karp HM, Skopintsev P, Thornton BW, Weissman RF, Yoon PH, Chen L, Hessler T, Eggers AR, Colognori D, Boger R, Doherty EE, Tsuchida CA, Tran RV, Hofman L, Shi H, Wasko KM, Zhou Z, Xia C, Al-Shimary MJ, Patel JR, Thomas VCJX, Pattali R, Kan MJ, Vardapetyan A, Yang A, Lahiri A, Maxwell MF, Murdock AG, Ramit GC, Henderson HR, Calvert RW, Bamert RS, Knott GJ, Lapinaite A, Pausch P, Cofsky JC, Sontheimer EJ, Wiedenheft B, Fineran PC, Brouns SJJ, Sashital DG, Thomas BC, Brown CT, Goltsman DSA, Barrangou R, Siksnys V, Banfield JF, Savage DF, Doudna JA. Adler BA, et al. Nucleic Acids Res. 2024 Jan 5;52(D1):D590-D596. doi: 10.1093/nar/gkad890. Nucleic Acids Res. 2024. PMID: 37889041 Free PMC article.
Robotic RNA extraction for SARS-CoV-2 surveillance using saliva samples.
Hamilton JR, Stahl EC, Tsuchida CA, Lin-Shiao E, Tsui CK, Pestal K, Gildea HK, Witkowsky LB, Moehle EA, McDevitt SL, McElroy M, Keller A, Sylvain I, Hirsh A, Ciling A, Ehrenberg AJ, Ringeisen BR, Huberty G, Urnov FD, Giannikopoulos P, Doudna JA; IGI SARS-CoV-2 Consortium. Hamilton JR, et al. PLoS One. 2021 Aug 5;16(8):e0255690. doi: 10.1371/journal.pone.0255690. eCollection 2021. PLoS One. 2021. PMID: 34351984 Free PMC article.
LuNER: Multiplexed SARS-CoV-2 detection in clinical swab and wastewater samples.
Stahl EC, Gopez AR, Tsuchida CA, Fan VB, Moehle EA, Witkowsky LB, Hamilton JR, Lin-Shiao E, McElroy M, McDevitt SL, Ciling A, Tsui CK, Pestal K, Gildea HK, Keller A, Sylvain IA, Williams C, Hirsh A, Ehrenberg AJ, Kantor R, Metzger M, Nelson KL, Urnov FD, Ringeisen BR, Giannikopoulos P, Doudna JA; IGI Testing Consortium. Stahl EC, et al. PLoS One. 2021 Nov 10;16(11):e0258263. doi: 10.1371/journal.pone.0258263. eCollection 2021. PLoS One. 2021. PMID: 34758033 Free PMC article.
Mitigation of chromosome loss in clinical CRISPR-Cas9-engineered T cells.
Tsuchida CA, Brandes N, Bueno R, Trinidad M, Mazumder T, Yu B, Hwang B, Chang C, Liu J, Sun Y, Hopkins CR, Parker KR, Qi Y, Satpathy AT, Stadtmauer EA, Cate JHD, Eyquem J, Fraietta JA, June CH, Chang HY, Ye CJ, Doudna JA. Tsuchida CA, et al. bioRxiv [Preprint]. 2023 Mar 22:2023.03.22.533709. doi: 10.1101/2023.03.22.533709. bioRxiv. 2023. Update in: Cell. 2023 Oct 12;186(21):4567-4582.e20. doi: 10.1016/j.cell.2023.08.041 PMID: 36993359 Free PMC article. Updated. Preprint.