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Use of thermal melt curves to assess the quality of enzyme preparations.
Crowther GJ, He P, Rodenbough PP, Thomas AP, Kovzun KV, Leibly DJ, Bhandari J, Castaneda LJ, Hol WG, Gelb MH, Napuli AJ, Van Voorhis WC. Crowther GJ, et al. Anal Biochem. 2010 Apr 15;399(2):268-75. doi: 10.1016/j.ab.2009.12.018. Epub 2009 Dec 14. Anal Biochem. 2010. PMID: 20018159 Free PMC article.
Structure determination of glycogen synthase kinase-3 from Leishmania major and comparative inhibitor structure-activity relationships with Trypanosoma brucei GSK-3.
Ojo KK, Arakaki TL, Napuli AJ, Inampudi KK, Keyloun KR, Zhang L, Hol WG, Verlinde CL, Merritt EA, Van Voorhis WC. Ojo KK, et al. Mol Biochem Parasitol. 2011 Apr;176(2):98-108. doi: 10.1016/j.molbiopara.2010.12.009. Epub 2010 Dec 30. Mol Biochem Parasitol. 2011. PMID: 21195115 Free PMC article.