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Bacterial genome engineering using CRISPR-associated transposases.
Gelsinger DR, Vo PLH, Klompe SE, Ronda C, Wang HH, Sternberg SH. Gelsinger DR, et al. Nat Protoc. 2024 Mar;19(3):752-790. doi: 10.1038/s41596-023-00927-3. Epub 2024 Jan 12. Nat Protoc. 2024. PMID: 38216671 Free PMC article. Review.
Proteolysis-targeting chimeras with reduced off-targets.
Nguyen TM, Sreekanth V, Deb A, Kokkonda P, Tiwari PK, Donovan KA, Shoba V, Chaudhary SK, Mercer JAM, Lai S, Sadagopan A, Jan M, Fischer ES, Liu DR, Ebert BL, Choudhary A. Nguyen TM, et al. Nat Chem. 2024 Feb;16(2):218-228. doi: 10.1038/s41557-023-01379-8. Epub 2023 Dec 18. Nat Chem. 2024. PMID: 38110475 Free PMC article.
Phage-assisted continuous and non-continuous evolution.
Miller SM, Wang T, Liu DR. Miller SM, et al. Nat Protoc. 2020 Dec;15(12):4101-4127. doi: 10.1038/s41596-020-00410-3. Epub 2020 Nov 16. Nat Protoc. 2020. PMID: 33199872 Free PMC article.
In vivo hypermutation and continuous evolution.
Molina RS, Rix G, Mengiste AA, Alvarez B, Seo D, Chen H, Hurtado J, Zhang Q, Donato García-García J, Heins ZJ, Almhjell PJ, Arnold FH, Khalil AS, Hanson AD, Dueber JE, Schaffer DV, Chen F, Kim S, Ángel Fernández L, Shoulders MD, Liu CC. Molina RS, et al. Nat Rev Methods Primers. 2022;2:37. doi: 10.1038/s43586-022-00130-w. Epub 2022 May 19. Nat Rev Methods Primers. 2022. PMID: 37073402 Free PMC article. No abstract available.
Efficient prime editing in two-cell mouse embryos using PEmbryo.
Kim-Yip RP, McNulty R, Joyce B, Mollica A, Chen PJ, Ravisankar P, Law BK, Liu DR, Toettcher JE, Ivakine EA, Posfai E, Adamson B. Kim-Yip RP, et al. Nat Biotechnol. 2024 Dec;42(12):1822-1830. doi: 10.1038/s41587-023-02106-x. Epub 2024 Feb 6. Nat Biotechnol. 2024. PMID: 38321114 Free PMC article.
24 results